htb63 human melanoma cell line Search Results


ht144  (ATCC)
95
ATCC ht144
Figure 1. miR‑675 expression is downregulated in melanoma tissues and cell lines. (A) Relative miR‑675 expression levels in 21 paired melanoma tissues and corresponding adjacent normal tissues were detected by RT‑qPCR anal ysis. *P<0.05 compared with adjacent normal tissues. (B) RT‑qPCR analysis of miR‑675 expression in four human melanoma cell lines (A375, A2058, <t>HT144</t> and SK‑MEL‑28) and the HEM. *P<0.05 compared with HEM. HEM, human epidermal melanocytes.
Ht144, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/htb63+human+melanoma+cell+line/HT-144/pm29257296-49-6-13
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human melanoma cell lines  (ATCC)
93
ATCC human melanoma cell lines
FIGURE 2. RT-PCR analysis of HLA-D gene transcription in the four <t>melanoma</t> <t>cell</t> <t>lines</t> and in the BUA cell line. The number of amplification cycles is given for each primer set in Table I. cDNAs either from cells treated for 48 h with 250 U/ml IFN-g (1) or from untreated cells (2) and the PCR products were analyzed on ethidium bromide-stained agarose gels. To assess that equal amounts of cDNA were used, a 16-cycle amplification was performed on the cDNAs with GAPDH primers, and the correspond- ing Dig-labeled PCR products were stained with CSPD.
Human Melanoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/htb63+human+melanoma+cell+line/HT-144%3B+Melanoma%3B+Human/pm11418637-64-10-8
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malignant ht 144 atcc htb 63 b16f10 atcc crl  (ATCC)
99
ATCC malignant ht 144 atcc htb 63 b16f10 atcc crl
FIGURE 2. RT-PCR analysis of HLA-D gene transcription in the four <t>melanoma</t> <t>cell</t> <t>lines</t> and in the BUA cell line. The number of amplification cycles is given for each primer set in Table I. cDNAs either from cells treated for 48 h with 250 U/ml IFN-g (1) or from untreated cells (2) and the PCR products were analyzed on ethidium bromide-stained agarose gels. To assess that equal amounts of cDNA were used, a 16-cycle amplification was performed on the cDNAs with GAPDH primers, and the correspond- ing Dig-labeled PCR products were stained with CSPD.
Malignant Ht 144 Atcc Htb 63 B16f10 Atcc Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/htb63+human+melanoma+cell+line/B16-F10/us10772893-2994-38-40
Average 99 stars, based on 1 article reviews
malignant ht 144 atcc htb 63 b16f10 atcc crl - by Bioz Stars, 2026-09
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Image Search Results


Figure 1. miR‑675 expression is downregulated in melanoma tissues and cell lines. (A) Relative miR‑675 expression levels in 21 paired melanoma tissues and corresponding adjacent normal tissues were detected by RT‑qPCR anal ysis. *P<0.05 compared with adjacent normal tissues. (B) RT‑qPCR analysis of miR‑675 expression in four human melanoma cell lines (A375, A2058, HT144 and SK‑MEL‑28) and the HEM. *P<0.05 compared with HEM. HEM, human epidermal melanocytes.

Journal: Molecular medicine reports

Article Title: MicroRNA‑675 inhibits cell proliferation and invasion in melanoma by directly targeting metadherin.

doi: 10.3892/mmr.2017.8264

Figure Lengend Snippet: Figure 1. miR‑675 expression is downregulated in melanoma tissues and cell lines. (A) Relative miR‑675 expression levels in 21 paired melanoma tissues and corresponding adjacent normal tissues were detected by RT‑qPCR anal ysis. *P<0.05 compared with adjacent normal tissues. (B) RT‑qPCR analysis of miR‑675 expression in four human melanoma cell lines (A375, A2058, HT144 and SK‑MEL‑28) and the HEM. *P<0.05 compared with HEM. HEM, human epidermal melanocytes.

Article Snippet: Melanoma cell lines, including A375, A2058, HT144 and SK-MEL-28, were obtained from the American Type Culture Collection (Manassas, VA, USA), and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% foetal bovine serum (FBS), 100 mg/ml penicillin and 100 mg/ml streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA).

Techniques: Expressing

Figure 2. miR‑675 overexpression inhibits cell proliferation and invasion in melanoma. A375 and HT144 cells were transfected with miR‑675 mimics or miR‑NC. (A) After transfection, RT‑qPCR was used to evaluate the transfection efficiency. *P<0.05 compared with miR‑NC. (B) MTT assays were performed to determine the proliferative ability in indicated cells. *P<0.05 compared with miR‑NC. (C) Cell invasion abilities in indicated cells were examined using Transwell invasion assays. *P<0.05 compared with miR‑NC. NC, negative control.

Journal: Molecular medicine reports

Article Title: MicroRNA‑675 inhibits cell proliferation and invasion in melanoma by directly targeting metadherin.

doi: 10.3892/mmr.2017.8264

Figure Lengend Snippet: Figure 2. miR‑675 overexpression inhibits cell proliferation and invasion in melanoma. A375 and HT144 cells were transfected with miR‑675 mimics or miR‑NC. (A) After transfection, RT‑qPCR was used to evaluate the transfection efficiency. *P<0.05 compared with miR‑NC. (B) MTT assays were performed to determine the proliferative ability in indicated cells. *P<0.05 compared with miR‑NC. (C) Cell invasion abilities in indicated cells were examined using Transwell invasion assays. *P<0.05 compared with miR‑NC. NC, negative control.

Article Snippet: Melanoma cell lines, including A375, A2058, HT144 and SK-MEL-28, were obtained from the American Type Culture Collection (Manassas, VA, USA), and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% foetal bovine serum (FBS), 100 mg/ml penicillin and 100 mg/ml streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA).

Techniques: Over Expression, Transfection, Negative Control

Figure 3. MTDH is a direct target of miR‑675 in melanoma. (A) Wild‑type (Wt) and mutant (Mut) of putative miR‑675 binding sequences in the 3'‑UTR of MTDH. (B) A375 and HT144 cells were cotransfected with miR‑675 mimics or miR‑NC and pGL3‑MTDH‑3'‑UTR Wt or pGL3‑MTDH‑3'‑UTR Mut. After 48 h of incubation, the luciferase activities were detected using the Dual‑Luciferase® Reporter Assay System. *P<0.05 compared with miR‑NC. (C) RT‑qPCR analysis of MTDH mRNA expression in A375 and HT144 cells after transfection with miR‑675 mimics or miR‑NC. *P<0.05 compared with miR‑NC. (D) Western blotting analysis was utilised to measure MTDH protein in A375 and HT144 cells after transfection with miR‑675 mimics or miR‑NC. *P<0.05 compared with miR‑NC. NC, negative control; MTDH, metadherin.

Journal: Molecular medicine reports

Article Title: MicroRNA‑675 inhibits cell proliferation and invasion in melanoma by directly targeting metadherin.

doi: 10.3892/mmr.2017.8264

Figure Lengend Snippet: Figure 3. MTDH is a direct target of miR‑675 in melanoma. (A) Wild‑type (Wt) and mutant (Mut) of putative miR‑675 binding sequences in the 3'‑UTR of MTDH. (B) A375 and HT144 cells were cotransfected with miR‑675 mimics or miR‑NC and pGL3‑MTDH‑3'‑UTR Wt or pGL3‑MTDH‑3'‑UTR Mut. After 48 h of incubation, the luciferase activities were detected using the Dual‑Luciferase® Reporter Assay System. *P<0.05 compared with miR‑NC. (C) RT‑qPCR analysis of MTDH mRNA expression in A375 and HT144 cells after transfection with miR‑675 mimics or miR‑NC. *P<0.05 compared with miR‑NC. (D) Western blotting analysis was utilised to measure MTDH protein in A375 and HT144 cells after transfection with miR‑675 mimics or miR‑NC. *P<0.05 compared with miR‑NC. NC, negative control; MTDH, metadherin.

Article Snippet: Melanoma cell lines, including A375, A2058, HT144 and SK-MEL-28, were obtained from the American Type Culture Collection (Manassas, VA, USA), and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% foetal bovine serum (FBS), 100 mg/ml penicillin and 100 mg/ml streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA).

Techniques: Mutagenesis, Binding Assay, Incubation, Luciferase, Reporter Assay, Expressing, Transfection, Western Blot, Negative Control

Figure 5. The upregulation of MTDH partially reverses the inhibition effects induced by miR‑675 in melanoma. (A) MTDH protein expression was analysed in A375 and HT144 cells transfected with miR‑675 mimics, with/without the MTDH‑overexpressing plasmid. *P<0.05 compared with miR‑NC. #P<0.05 compared with miR‑675 mimics+pcDNA3.1‑MTDH. Cell proliferation (B) and invasion (C) were determined in A375 and HT144 cells transfected with miR‑675 mimics with or without pcDNA3.1‑MTDH. *P<0.05 compared with miR‑NC. #P<0.05 compared with miR‑675 mimics+pcDNA3.1‑MTDH. NC, negative control; MTDH, metadherin.

Journal: Molecular medicine reports

Article Title: MicroRNA‑675 inhibits cell proliferation and invasion in melanoma by directly targeting metadherin.

doi: 10.3892/mmr.2017.8264

Figure Lengend Snippet: Figure 5. The upregulation of MTDH partially reverses the inhibition effects induced by miR‑675 in melanoma. (A) MTDH protein expression was analysed in A375 and HT144 cells transfected with miR‑675 mimics, with/without the MTDH‑overexpressing plasmid. *P<0.05 compared with miR‑NC. #P<0.05 compared with miR‑675 mimics+pcDNA3.1‑MTDH. Cell proliferation (B) and invasion (C) were determined in A375 and HT144 cells transfected with miR‑675 mimics with or without pcDNA3.1‑MTDH. *P<0.05 compared with miR‑NC. #P<0.05 compared with miR‑675 mimics+pcDNA3.1‑MTDH. NC, negative control; MTDH, metadherin.

Article Snippet: Melanoma cell lines, including A375, A2058, HT144 and SK-MEL-28, were obtained from the American Type Culture Collection (Manassas, VA, USA), and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% foetal bovine serum (FBS), 100 mg/ml penicillin and 100 mg/ml streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA).

Techniques: Inhibition, Expressing, Transfection, Plasmid Preparation, Negative Control

FIGURE 2. RT-PCR analysis of HLA-D gene transcription in the four melanoma cell lines and in the BUA cell line. The number of amplification cycles is given for each primer set in Table I. cDNAs either from cells treated for 48 h with 250 U/ml IFN-g (1) or from untreated cells (2) and the PCR products were analyzed on ethidium bromide-stained agarose gels. To assess that equal amounts of cDNA were used, a 16-cycle amplification was performed on the cDNAs with GAPDH primers, and the correspond- ing Dig-labeled PCR products were stained with CSPD.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 2. RT-PCR analysis of HLA-D gene transcription in the four melanoma cell lines and in the BUA cell line. The number of amplification cycles is given for each primer set in Table I. cDNAs either from cells treated for 48 h with 250 U/ml IFN-g (1) or from untreated cells (2) and the PCR products were analyzed on ethidium bromide-stained agarose gels. To assess that equal amounts of cDNA were used, a 16-cycle amplification was performed on the cDNAs with GAPDH primers, and the correspond- ing Dig-labeled PCR products were stained with CSPD.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Staining, Labeling

FIGURE 1. Cell surface expression of the HLA-DR molecules in the 42/95 and HT144 melanoma cell lines examined by indirect immunoflu- orescence. The cells were either treated with 250 U/ml IFN-g for 48 h or untreated as indicated on the figure (light histograms). The dark histogram corresponds to the isotypic control. The L243 mAb was used to detect the expression of HLA-DR molecules by cytofluorometry.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 1. Cell surface expression of the HLA-DR molecules in the 42/95 and HT144 melanoma cell lines examined by indirect immunoflu- orescence. The cells were either treated with 250 U/ml IFN-g for 48 h or untreated as indicated on the figure (light histograms). The dark histogram corresponds to the isotypic control. The L243 mAb was used to detect the expression of HLA-DR molecules by cytofluorometry.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Expressing, Control

FIGURE 4. RT-PCR analysis of CIITA transcript expression in the dif- ferent melanoma cell lines and in the BUA cell line through a 33-cycle amplification procedure. cDNAs were prepared from cells either untreated (2) or treated (1) with 250 U/ml IFN-g for 6 h. The amplification products were detected on ethidium bromide-stained gels. To assess that equal amounts of cDNAs were used, a 16-cycle amplification was performed on the cDNAs with GAPDH-specific primers, and the Dig-labeled amplifica- tion products were stained with CSPD.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 4. RT-PCR analysis of CIITA transcript expression in the dif- ferent melanoma cell lines and in the BUA cell line through a 33-cycle amplification procedure. cDNAs were prepared from cells either untreated (2) or treated (1) with 250 U/ml IFN-g for 6 h. The amplification products were detected on ethidium bromide-stained gels. To assess that equal amounts of cDNAs were used, a 16-cycle amplification was performed on the cDNAs with GAPDH-specific primers, and the Dig-labeled amplifica- tion products were stained with CSPD.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Staining, Labeling

FIGURE 5. RT-PCR analysis of IRF-1 transcript expression in the dif- ferent melanoma cell lines and in the BUA cell line. cDNA quantification with GAPDH-specific primers was performed on Dig-labeled amplified products.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 5. RT-PCR analysis of IRF-1 transcript expression in the dif- ferent melanoma cell lines and in the BUA cell line. cDNA quantification with GAPDH-specific primers was performed on Dig-labeled amplified products.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Labeling